DBCO NHS ester

Dibenzocyclooctyne (ADIBO, DBCO) is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (spAAC) – a copper free Click chemistry reaction.

This is an amine-reactive NHS ester that provides easy attachment of the reactive moiety to almost any primary or secondary amine group, such as that of protein, peptide, or small molecule amine.

DBCO reacts instantly with azides. The rate of the reaction is much higher than that of copper-catalyzed reaction, and reactions with many other cyclooctynes. Unlike some other cyclooctynes, DBCO does not react with tetrazines - this allows to carry out orthogonal conjugation of azides with DBCO, and trans-cyclooctenes with tetrazines.

General properties

Appearance:                                                      off white solid

Mass spec M+ increment:                                 315.1

Molecular weight:                                               430.45

Molecular formula:                                             C25H22N2O5

IUPAC name:                                                     6-{2-Azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl}-6-oxohexanamide

Solubility:                                                            good in DCM, DMF, DMSO

Quality control:                                                   NMR 1H, HPLC-MS (95%)

Storage conditions:Storage:                           12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.