上海芃硕生物科技有限公司

（备注：非常规分子量：400、600。精确聚合度的小分子定制。）

结构式：

基本信息：

中文名称：Dibenzocyclooctyne-聚乙二醇-羧基 (DBCO-PEG-COOH)

别名：DBCO-PEG-COOH

英文名称：DBCO-PEG-COOH，DBCO-PEG-ACID

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

纯度：95%以上

应用简介：

Ponsure’DBCO(dibenzocyclooctyne) PEG acid, DBCO-PEG-COOH, is a heterobifunctional reactivePEG derivative that can go Click Chemistry reaction without a need of any metalcatalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes andazides, also termed as the Cu-free click reaction, is a bioorthogonal reactionthat enables the conjugation of two molecules in aqueous solution. DBCO PEG derivativespossess fast kinetics and stability in aqueous buffer. DBCO reagents can beused to label azide-modified biomolecules spontaneously without the need fortoxic Cu catalysts. Carboxylic acid group, on the other hand, can be activatedto react with amines, hydroxy and other functional groups.

Copper-freeClick reaction procedures:

Preparethe azide-containing reagents in reaction buffer.

AddDBCO reagents to azide containing reagents. Add 1 mol equivalent of limitingreagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubatethe reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.

Purifyconjugates either by dialysis or size-exclusion chromatograph.

物理性质：

状态：淡黄色到白色固体，或者半固体，取决于分子量。

溶解性：溶于水，氯仿，DMSO等溶剂。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

反应性：DBCO于含有N3基团的反应。NHS可以和NH2。

技术指标：

纯度（PEG）：95%以上

取代率（DBCO）：90%以上

取代率（COOH）：95%以上

分散系数（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF