上海芃硕生物科技有限公司

（备注：非常规分子量：400、600。精确聚合度的小分子定制。）

结构式：

基本信息：

中文名称：Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

别名：FITC-PEG-DBCO

英文名称：FITC-PEG-DBCO，Fluorescein-PEG-DBCO

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

纯度：90%以上

应用简介：

DBCO(Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides viastrain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonalreaction also termed Cu-free click reaction. This reaction has excellent selectivity and biocompatibility, such thatthe complimentary reagents can form covalent bonds with richly functionalizedbiological systems. The Cu-free click reaction has been a powerful tool incatalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stabilityin aqueous buffer and they can be used to label azide-modified biomoleculeswith high specificity and reactivity.

Copper-freeClick Reaction Procedures:

Preparethe azide-containing sample in reaction buffer. Add DBCO conjugate to azidecontaining sample. Recommendation: Add 1 mole equivalent of limiting reagent to1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction atroom temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reactionis now ready for purification.

物理性质：

状态：淡黄色到黄色固体，或者半固体，取决于分子量。

溶解性：溶于DMSO,DMF等溶剂。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

反应性：DBCO于含有N3基团的反应。

技术指标：

纯度（PEG）：90%以上

取代率（DBCO）：90%以上

取代率（FITC）：90%以上

分散系数（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF