上海芃硕生物科技有限公司

（备注：非常规分子量：400、600。精确聚合度的小分子定制。）

结构式：

基本信息：

中文名称：Dibenzocyclooctyne-聚乙二醇-马来酰亚胺 (DBCO-PEG-MAL)

别名：DBCO-PEG-Maleimide

英文名称：DBCO-PEG-Maleimide，DBCO-PEG-MAL

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

纯度：95%以上

应用简介：

MaleimidePEG DBCO (dibenzocycolctyne) is one of Ponsure DBCO PEG derivatives that can goClick Chemistry reaction without a need of any metal catalysts. Thestrain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, alsotermed as the Cu-free click reaction, is a bioorthogonal reaction that enablesthe conjugation of two molecules in aqueous solution. DBCO PEG derivativespossess fast kinetics and stability in aqueous buffer. DBCO reagents can beused to label azide-modified biomolecules spontaneous without the need fortoxic Cu catalysts. Meanwhile, maleimide group can react with sulfhydryl(Thoil) groups at pH 6.5-7.5 easily. PEGylation can increase solubility andstability and reduce immunogenicity of peptides and proteins. It can alsosuppress the non-specific binding of charged molecules to the modifiedsurfaces.

Copper-freeClick Reaction Procedures:

Preparethe azide-containing reagents in reaction buffer.

AddDBCO reagents to azide containing reagents. Add 1 mol equivalent of limitingreagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubatethe reaction at room temperature for 2~4 hours or 2~12 hours at 4℃.

Purifyconjugates either by dialysis or size-exclusion chromatograph.

物理性质：

状态：淡黄色到黄色固体，或者半固体，取决于分子量。

溶解性：溶于DMSO,DMF等溶剂。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

反应性：DBCO于含有N3基团的反应。MAL可以和SH。

技术指标：

纯度（PEG）：95%以上

取代率（DBCO）：90%以上

取代率（MAL）：90%以上

分散系数（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF